SELECTED PUBLICATIONS

Matrix and Metalloproteinase Biology

Pal, S., Chen, Z., Xu, X., Mikhailova, M., Steffensen, B.  Co-purified gelatinases alter the stability and biological activities of human plasma fibronectin.  J. Periodont. Res.  (in press; Accepted June 2009) 

Xu, X., Mikhailova, M., Chen, Z., Ilangovan, U., Chen, Z., Yu, A., Pal, S. Hinck, A.P, Steffensen, B.  Nuclear magnetic resonance mapping and functional confirmation of the collagen binding sites of matrix metalloproteinase-2.  Biochemistry 48(25): 5822-5831, 2009

Johnson, D.L., Carnes, D., Steffensen, B., Cochran, D.L.  Cellular effects of enamel matrix derivative are associated with specific protein components.  J. Periodontol. 80: 648-656, 2009

Murillo, J., Wang, Y., Xu, X., Klebe, R.J., Chen, Z., Zardeneta, G., Pal, S., Mikhailova, M., Steffensen, B.  Advanced glycation of type I collagen and fibronectin modifies periodontal cell behavior.   J. Periodontol. 79: 2190-2199, 2008.

Xu, X., Chen, Z., Wang, Y., Bonewald, L., Steffensen, B.  Inhibition of MMP-2 gelatinolysis by targeting exodomain-substrate interactions.  Collagen-like peptide blocks gelatin positioning by the collagen-binding domain.  Biochem. J. 406: 147-155, 2007.

Cortez, D.M.,Feldman, M.D., Mummidi, S., Valente, A.J., Steffensen, B., Vicenti, M., Barnes, J.L., Chandrasekar, B.  IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta, NF-kappaB, and AP-1 activation. Am. J. Physiol. Heart Circ. Physiol. 293(6): H3356-H3365, 2007.

Xu, X., Chen, Z., Wang, Y., Yamada, Y., Steffensen, B.  Functional basis for the overlap in MMP-2 and MMP-9 ligand interactions and substrate Specificities.  Biochem J. 392: 127-134, 2005.

Oates, T.W., Maller, S., Steffensen, B.  Differential gingival fibroblast integrin expression as a function of titanium surfaces characteristics.  J. Periodontol. 76(10): 1743-1750, 2005.

Xu, X., Wang, Y., Chen, Z., Sternlicht, M.D., Hidalgo, M., Steffensen, B.  Matrix metalloproteinase-2 contributes to cancer cell migration on collagen.  Cancer Research 65(1): 123-131, 2005.

Xu, X., Wang, Y., Lauer-Fields, J.L., Fields, G.B., Steffensen, B.  Contributions of the MMP-2 collagen binding domain to gelatin cleavage.  Substrate binding via the collagen binding domain is required for MMP-2 degradation of gelatin but not short peptides.  Matrix Biol. 23: 171-181, 2004.

Steffensen, B., Xu, X., Martin, P.A., Zardeneta, G.  Human fibronectin and MMP-2 collagen binding domains compete for collagen binding sites and modify cellular activation of MMP-2.  Matrix Biol. 21(5): 399-414, 2002.

Hoang, A.M., Klebe, R.J., Steffensen, B., Ryu, O.H., Simmer, J.P., Cochran,D.L.  Amelogenin is a cell adhesion protein.  J. Dent. Res. 81(7): 497-500, 2002. with separate Appendix.

Steffensen, B., Häkkinen, L., and Larjava, H.S. Proteolytic events of wound healing: Coordinated interactions between matrix metalloproteinases (MMPs), integrins, and extracellular matrix molecules. Crit. Rev. Oral Biol. Med. 12: 373-398, 2001.

Steffensen, B., and Martin, J.A.: Fibronectin collagen binding domain (modules I6, II1, II2, I7) cDNA sequence. Gen Bank submission AF312399, October 10, 2000.

Overall, C.M., Wallon, U.M., Steffensen, B., De Clerck, Y., Tschesche, H., Abbey, R.S.: Substrate and TIMP interactions with human gelatinase A recombinant COOH-terminal hemopexin-like and fibronectin type II-like domains. In Edwards, D.A., Hawkes, S., Kokha, R. (Eds.) Metalloproteinases in Development and Disease. Gordon & Breach, Amsterdam, Holland, 2000, pp. 57-69.

Steffensen, B., Bigg, H. F., Overall, C. M.: The involvement of the fibronectin type II-like modules of human gelatinase A in cell surface localization and activation. J. Biol. Chem. 273: 20622-20628, 1998.

Bigg, H.F., Shi, Y.E., Liu, Y.E., Steffensen, B., Overall, C.M.: Specific, high-affinity binding of tissue inhibitor of metalloproteinases-4 (TIMP-4) to the COOH-terminal hemopexin-like domain of human gelatinase A: TIMP-4 binds progelatinase A and the COOH-terminal domain in a similar manner to TIMP-2. J. Biol. Chem. 272:15496-15500, 1997.

Steffensen, B., Wallon, U.M., Overall, C.M.: Extracellular matrix binding properties of recombinant fibronectin type II-like modules of 72-kDa gelatinase/type IV collagenase. High affinity binding to native type I collagen but not native type IV collagen. J. Biol. Chem. 19:11555-566, 1995.

Chen, D., Magnuson, V.L., Hill, S., Arnaud, C., Steffensen, B., Klebe, R.J.: Regulation of integrin expression by substrate adherence. J. Biol. Chem. 267(33):23502-23506, 1992.

Steffensen, B., Magnuson, V.L., Potempa, C.L., Chen, D., Klebe, R.J.: Alpha-5 integrin subunit expression changes during myogenesis. Biochem Biophys Acta, 1137: 95-100, 1992

Steffensen, B., Duong, A.H., Milam, S.B., Potempa, C.L., Magnuson, V.L., Klebe, R.J.: Immunohistochemical localization of cell adhesion proteins and integrins in the periodontium. J. Periodontol. 63:584-592, 1992.

Klebe, R.J., Overfelt, T.M., Magnuson, V.L., Steffensen, B., Chen, D., and Zardeneta, G.: The morphogenesis of fibroblast organizing centers involves extracellular matrix components and G-proteins. Proc. Natl. Acad. Sci. USA 88:9588-9592, 1991.

Milam, S.B., Steffensen, B., Haskin C., Zardeneta, G., Chen, D., Magnuson, V.L., Klebe, R.J.: Cell Adhesion Proteins in Oral Biology. CRC Crit. Rev. Oral Biol. Oral Med. 2:451-493, 1991.


Clinical Research

Stanley, C.M., Wang, Y., Pal, S., Klebe, R.J., Harkless, L.B., Xu, X., Chen, Z., Steffensen, B.  Fibronectin fragmentation is a feature of periodontal disease sites and diabetic foot and leg wounds and modifies cell behavior.  J. Peridontol. 79: 861-875, 2008.

Wang, Y., Morlandt, A., Xu, X., Carnes, D.L., Chen, Z., Steffensen, B.  Tetracycline at subcytotoxic levels inhibits MMP-2 and -9 but does not remove the smear layer.  J. Peridontol. 76(7): 1129-1139, 2005.

Steffensen, B., Sottosanti, J.: Treating Plaque-associated Gingivitis. In Wilson, T.G., Kornman, K.S. (Eds.) Fundamentals of Periodontics. 2nd edition.  Quintessence Publishing Co., Chicago 2003.

Nummikoski, P.V., Steffensen, B., Hamilton, K., Dove, S.B.: Clinical validation of a new subtraction radiography technique (DSRÔ ) for periodontal bone loss detection. J. Periodontol  71(4): 598-605, 2000.

Steffensen, B., Sottosanti, J.: Treating Plaque-associated Gingivitis. In Wilson, T.G., Kornman, K.S. (Eds.)  Fundamentals of Periodontics. 1st edition. Quintessence Publishing Co., Chicago 1996.

Schetritt, A., Steffensen, B.: Diagnosis and management of vertical root fractures. J. Canada Dent. Assoc. 61: 607-613, 1995

Fayad, S., Steffensen, B.: Root resorptions in a patient with hemifacial atrophy. J. Endodont. 20: 299-303, 1994

Ebersole, J.L., Singer, R.E., Steffensen, B., Filloon, T., Kornman, K.S.: Inflammatory mediators and immunoglobulins in GCF from healthy, gingivitis and periodontitis sites.  J. Periodont. Res. 28: 543-546, 1993

Guillemin, M.R., Mellonig, J.T., Brunsvold, M.A., Steffensen, B.: Healing in periodontal defects treated by decalcified freeze-dried bone allografts in combination with PTFE membranes.  J. Clin. Periodontol. 20: 520-527, 1993

Steffensen, B., Storey, A.T.: Effects of orthodontic intrusive forces in treatment of periodontally compromised incisors. Case Report. Int. J. Periodont. Restor. Dent. 13: 433-441, 1993

Ebersole, J.L., Brunsvold, M., Steffensen, B., Wood, R., Holt, S.C.: Effects of immunization with Porphyromonas gingivalis and Prevotella intermedia on progression of ligature-induced periodontitis in the nonhuman primate, Macaca fascicularis. Infect. Immun. 59:3351-3359, 1991.

Steffensen, B., Brägger, U., Yuan, C., Pasquale, L.A., Brunsvold, M., Kornman, K.S.: Effect of region of interest in computerized densitometric analysis of radiographs. J. Periodont. Res. 26:107-113, 1991.

Steffensen, B., Pasquali, L.A., Yuan, C., Wood, R.C., Schoolfield, J.D., Kornman, K.S.: Correction of density changes caused by methodological errors in CADIA. J. Periodont. Res. 24:402-408, 1989.

Steffensen, B., Weber, H.-P.: Relationship between the radiographic defect angle and healing after treatment. J. Periodontol. 60:248-254, 1989.

Helldén, L., Elliott, A., Steffensen, B., Steffensen, J.E.M.: The prognosis of tunnel preparations in treatment of class III furcations. J. Periodontol. 60:182-187, 1989.


Recent Laboratory Abstract Presentations

Yuan, G., Yang, G. Gonzales, C., Gluhak-Heinrich, J., Xu, X., Chen, Z., Steffensen, B., Donly, K., MacDougall, M., Chen, Z., Chen, S.  Tooth defects and DSP/DSPP processing interference in MMP-9 knock-out mice.  J. Dent. Res. 88 (Spec. Iss. A) #16922, 2009.

Mikhailova, M., Xu, X., Chen, Z., Pal, S., Steffensen, B.  Identical amino acids on MMP-2 position different types of collagen for cleavage.  The 27th Annual Dental Science Symposium, UTHSCSA, 2009 and The Cancer Development and Progression Program Retreat, San Antonio, 2009. 

Xu, X., Baber, S., Chen, Z., Pal, S., Mikhailova, M., Steffensen, B.  Inhibition of proMMP-2 activation by carboxyl-terminal domain of TIMP-2 in vitro.  The American Society for Cell Biology Annual Meeting Special Poster Session, Abstr. #L65, Washington, D.C., 2008.
Also presented at The 27th Annual Dental Science Symposium, UTHSCSA, 2009 and The Cancer Development and Progression Program Retreat, San Antonio, 2009

Pal, S., Chen, Z., Xu, X., Mikhailova, M., Wang, Y., Steffensen, B.  Cleavage of fibronectin by MMP-2 generates fragments with distinct biological activities.  The American Society for Cell Biology Annual Meeting Special Poster Session, Abstr. #L63, Washington, D.C., 2008.
Also presented at The 27th Annual Dental Science Symposium, UTHSCSA, 2009 and The Cancer Development and Progression Program Retreat, San Antonio, 2009

Baber, S., Steffensen, B., Chen, Z., Mikhailova, M., Pal, S., Xu, X.  Competitive inhibition of proMMP-2 activation.  J. Dent. Res. 87 (Spec. Iss. A) #1012, 2008.
Also presented at The 26th Annual Dental Science Symposium, UTHSCSA, 2008

Chen, Z., Xu, X., Pal, S., Mikhailova, M.,  Su, J., Steffensen, B.  Contributions of the Hemopexin-like Domain of MMP-2 to Fibronectin Cleavage.  The 26th Annual Dental Science Symposium, UTHSCSA, 2008. 

Xu, X., Chen, Z., Mikhailova, M., Pal, S., Steffensen, B.  Investigating Mechanisms of Cellular Activation of Pro-MMP-2 by Recombinant Protein Engineering.  The 26th Annual Dental Science Symposium, UTHSCSA, 2008. 

Pal, S., Chen, Z., Xu, X., Mikhailova, M., Steffensen, B. Cell Attachment Properties of Fibronectin Fragments Generated by MMP-2.  The 26th Annual Dental Science Symposium, UTHSCSA, 2008. 

Mikhailova, M., Xu, X., Chen, Z., Pal, S., Steffensen, B.  Cooperativity of Collagen-binding Domain Modules is Essential for MMP-2 Gelatinolytic Activities. The 26th Annual Dental Science Symposium, UTHSCSA, 2008. 

Pal, S.,. Chen, Z., Xu, X., Mikhailova, M., Klebe, R.J., Steffensen, B.:  Enhanced strategy for separating human plasma fibronectin from MMPs by affinity chromatography.  The American Society for Cell Biology Annual Meeting Special Poster Session, Abstr. #2631, Washington, D.C., 2007.
Also presented at The UTHSCSA Department of BiochemistryAnnual Retreat, Fair Oaks, 2007

Steffensen, B., Stanley, C.M., Wang, Y., Pal, S., Klebe, R.J., Harkless, L.B., Xu, X., Chen, Z.:  Fibronectin-fragmentation is a feature of both periodontal disease sites and diabetic foot and leg wounds and modifies cell behavior.  The American Society for Cell Biology Annual Meeting Special Poster Session, Abstr. #2865, Washington, D.C., 2007.

Steffensen, B., Xu, X.,  Chen, Z.,  Wang, Y.,  Bonewald, L.:  Inhibition of MMP-2 by Targeting Exodomain-Substrate Interactions.  Gordon Research Conference on Matrix Metalloproteinases, Il Ciocco, Italy, 2007. (unpublished)

Chang, T.H., Steffensen, B., Alderete, J.F.:  Fibronectin ligand on Trichomonas vaginalis is surface-associated GAPDH.  Amer. Soc. Microbiol. 2007.

Wong, D., Steffensen, B., Steffensen, J.E.M., Mealey, B., Powell, C., Schoolfield, J., Eddy, S., Cappelli, D.:  Measuring the validity of a periodontal health self report.  J. Dent. Res. 86 (Spec. Iss. A) #638, 2007.

Montgomery, R., Steffensen, B., Chen, Z., Yu, A., Pal, S., Kalmykov, E., Xu, X.:  Cloning and expression of Treponema denticola fibronectin-binding protein (Fbp).  J. Dent. Res. 86 (Spec. Iss A) #2873, 2007.

Chung, M., Carnes, D. , Steffensen, B. ,  Cochran, D.:  Enamel matrix derivative stimulates MMP-2 and -14 expression by PDL and vascular cells.  J. Dent. Res. 86 (Spec. Iss. A) #2373, 2007.